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KMID : 0613820080180020162
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Journal of Life Science
2008 Volume.18 No. 2 p.162 ~ p.166
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Optimization of the Medium Composition for Heteropolysaccharide-7 Production by Beijerinckia indica L3 Using Response Surface Methodology
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Ra Chae-Hun
Kim Ki-Myong
Huh Pil-Woo
Choi Mi-Ran
Kim Sung-Koo
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Abstract
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The production of heteropolysaccharide-7 (PS-7) by Beijerinckia indica (B. indica L3) was evaluated in shaker flask culture. The medium optimization was studied using response surface methodology (RSM). A five-level three-factor central composite design was employed to determine the maximum PS-7 yield at optimum levels for whey lactose, glucose and ammonium nitrate contents. The validity of the model could be determined by the regression coefficient, . The values of were 0.72, 0.64 and 0.85 in PS-7, DCW and viscosity, respectively. The optimal medium combinations of whey lactose, glucose and ammonium nitrate concentrations on the PS-7 production were whey lactose (2%), glucose (1 %) and ammonium nitrate 5 mM, respectively. The result indicated that PS-7 production was affected significantly by the addition of glucose to whey lactose based on medium and C/N ratio.
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KEYWORD
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Beijerinckia indica L3, heteropolysaccharide-7, whey lactose
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